gcvH [2018-03-18 11:57:07]

glycine cleavage system protein H, lipoyl-binding site, octanoyltransferase for lipoic acid biosynthesis, required for lipoate transfer to 2-oxo acid dehydrogenases

locus: BSU32800

pI: 3.89

mw: 14.11 kDa

protein length: 127 aa

gene length: 381 bp

function: glycine utilization

product: glycine cleavage system protein H

essential: no

synonyms: yusH

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.8|Utilization of threonine/ glycine]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.7|Biosynthesis and scavenging of lipoic acid]

Gene

Coordinates: 3,366,123 → 3,366,506

The protein

Catalyzed reaction/ biological activity

• degradation of glycine. [protein|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|GcvH] shuttles the methylamine group of glycine from [protein|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|GcvPB] to [protein|F6F449170276055E6D60D89E9E17A152A82CA166|GcvT] (according to UniProt)
• assembly of lipoate, and transfer of the cofactor to

Protein family

• gcvH family (according to Swiss-Prot)

[SW|Cofactors]

• lipoic acid (on Lys-63), lipoylated by [protein|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|LipM] [Pubmed|20882995], can probably be removed by [protein|A0A6CB19191A251BB5600C18E039978A9A34933C|SrtN] [pubmed|28900027]

Structure

• [PDB|3IFT] (from ''Mycobacterium tuberculosis'', 50% identity) [Pubmed|20364333]

Expression and Regulation

Operons

• genes: [gene|5278DD377B110CC14E239B9428F1985B472EEDF5|yusI]-[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]-[gene|0253D9B030630340551DC2D4F40B3B118DB0EC56|yusG]
  description: [Pubmed|22383849]

• genes: [gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]-[gene|0253D9B030630340551DC2D4F40B3B118DB0EC56|yusG]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B592 (yusH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1591 NBRP B. subtilis, Japan]
• BKE32800 (Δ[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
• BKK32800 (Δ[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
• GP2585 (Δ[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]::tet comIQ12L) (in DK1042) available at [SW|Jörg Stülke]'s lab

References

Reviews

• 27074917

Original publications

• 20882995,21338421,20364333,28900027,29339506